PUBLICATIONS

Cryo-EM/ET in innate immunity 

S Zhu, CJ Bradfield, A Maminska, E Park, B Kim, P Kumar, S Huang, Y Zhang, J Bewersdorf, JD MacMicking “Native architecture of a human GBP1 defense complex for cell-autonomous immunity to infection" Science 383 (6686), 2024. DOI: 10.1126/science.abm9903

S Huang, S Zhu, P Kumar, JD MacMicking, “A phase-separated nuclear GBPL circuit controls immunity in plants,” Nature 594 (7863), 424-429, 2021.

RG Gaudet, S Zhu, A Halder, BH Kim, CJ Bradfield, A Maminska, TN Nguyen, M Lazarou, E Karatekin, K Gupta, JD MacMicking, “A human apolipoprotein L with detergent-like activity kills intracellular pathogens,” Science 373 (6552), 2021.

Cryo-ET in bacterial cell biology

BL Carroll, T Nishikino, W Guo, S Zhu, S Kojima, M Homma, J Liu, “The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching,” elife, 9:e61446, 2020.

H Zhang, GA Mulholland, S Seef, S Zhu, J Liu, T Mignot, B Nan, “Establishing rod shape from spherical, peptidoglycan-deficient bacterial spores,” Proc. Natl. Acad. Sci. U.S.A., vol. 117, no. 25, pp. 14444–14452, 2020.

K. A. Floyd, C. K. Lee, W. Xian, M. Nametalla, A. Valentine, B. Crair, S. Zhu, H. Q. Hughes, J. L. Chlebek, and D. C. Wu, “c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae,” Nat Commun, vol. 11, no. 1, pp. 1–16, 2020.

S. Zhu, T. Nishikino, N. Takekawa, H. Terashima, S. Kojima, K. Imada, M. Homma, and J. Liu, “In Situ Structure of the Vibrio Polar Flagellum Reveals a Distinct Outer Membrane Complex and Its Specific Interaction with the Stator,” Journal of Bacteriology, vol. 202, no. 4, 2020.

N. Takekawa, M. Isumi, H. Terashima, S. Zhu, Y. Nishino, M. Sakuma, S. Kojima, M. Homma, and K. Imada, “Structure of Vibrio FliL, a new stomatin like protein that assists the bacterial flagellar motor function,” MBio, vol. 10, no. 2, pp. e00292–19, 2019.

C. Chang, C. Wu, J. Osipiuk, S. D. Siegel, S. Zhu, X. Liu, A. Joachimiak, R.T. Clubb, A. Das, and H. Ton-That, “Cell-to-cell interaction requires optimal positioning of a pilus tip adhesin modulated by gram-positive transpeptidase enzymes,” Proc. Natl. Acad. Sci. U.S.A., vol. 116, no. 36, pp. 18041–18049, 2019.

S. Zhu, M. Schniederberend, D. Zhitnitsky, R. Jain, J. E. Galan, B. I. Kazmierczak, and J. Liu, “In situ structures of polar and lateral flagella revealed by cryo-electron tomography,” Journal of Bacteriology, vol. 201, no. 13, pp. e00117–19, 2019.

J. K. Chu, S. Zhu, C. M. Herrera, J. C. Henderson, J. Liu, M. S. Trent, and T. R. Hoover, “Loss of a cardiolipin synthase in Helicobacter pylori G27 blocks flagellum assembly,” Journal of Bacteriology, vol. 201, no. 21, pp. e00372–19, 2019.

S. Zhu, T. Nishikino, S. Kojima, M. Homma, and J. Liu, “The Vibrio H-ring facilitates the outer membrane penetration of the polar sheathed flagellum,” Journal of Bacteriology, vol. 200, no. 21, 2018.

T.-S. Lin, S. Zhu, S. Kojima, M. Homma, and C.-J. Lo, “FliL association with flagellar stator in the sodium-driven Vibrio motor characterized by the fluorescent microscopy,” Nature Publishing Group, vol. 8, no. 1, pp. 1–12, 2018.

S. Zhu, T. Nishikino, B. Hu, S. Kojima, M. Homma, and J. Liu, “Molecular architecture of the sheathed polar flagellum in Vibrio alginolyticus,” Proc. Natl. Acad. Sci. U.S.A., vol. 114, no. 41, pp. 10966–10971, 2017.

S. Zhu, Z. Qin, J. Wang, D. R. Morado, and J. Liu, “In situ structural analysis of the spirochetal flagellar motor by cryo-electron tomography,” pp. 229–242, 2017.

Z. Qin, W.-T. Lin, S. Zhu, A. T. Franco, and J. Liu, “Imaging the motility and chemotaxis machineries in Helicobacter pylori by cryo-electron tomography,” Journal of Bacteriology, vol. 199, no. 3, pp. e00695–16, 2017.

Molecular Microbiology and Biophysics

A. Kumar, M. Isumi, M. Sakuma, S. Zhu, Y. Nishino, Y. Onoue, S. Kojima, Y. Miyanoiri, K. Imada, and M. Homma, “Biochemical characterization of the flagellar stator-associated inner membrane protein FliL from Vibrio alginolyticus,” Journal of Biochemistry, vol. 161, no. 4, pp. 331–337, 2017.

T. Nishikino, S. Zhu, N. Takekawa, S. Kojima, Y. Onoue, and M. Homma, “Serine suppresses the motor function of a periplasmic PomB mutation in the Vibrio flagella stator,” Genes to Cells, vol. 21, no. 5, pp. 505–516, 2016.

S. Zhu, A. Kumar, S. Kojima, and M. Homma, “FliL associates with the stator to support torque generation of the sodium-driven polar flagellar motor of Vibrio,” Molecular Microbiology, vol. 98, no. 1, pp. 101–110, 2015.

S. Zhu, M. Takao, N. Li, M. Sakuma, Y. Nishino, M. Homma, S. Kojima, and K. Imada, “Conformational change in the periplasmic region of the flagellar stator coupled with the assembly around the rotor,” Proc. Natl. Acad. Sci. U.S.A., vol. 111, no. 37, pp. 13523–13528, 2014.

S. Zhu, S. Kojima, and M. Homma, “Structure, gene regulation and environmental response of flagella in Vibrio,” Front Microbiol, vol. 4, p. 410, 2013.

S. Zhu, M. Homma, and S. Kojima, “Intragenic suppressor of a plug deletion nonmotility mutation in PotB, a chimeric stator protein of sodium-driven flagella,” Journal of Bacteriology, vol. 194, no. 24, pp. 6728–6735, 2012.